Discovering macromolecular shape and interactions

Using Small angle X-ray scattering (SAXS), the BioXolver collects
structural information on biological samples.

The BioXolver can obtain key structural parameters, such as:

• radius of gyration
• molecular weight
• maximum intra-particle distance
• degree of folding
• low-resolution 3D protein shape.

Read more on SAXS for Structural Biology

In-lab SEC-SAXS, protein-protein interactions...

Discover our application notes :

High-throughput 3D protein envelope

Thorough investigation of oligomers during protein fibrillation

Protein-protein interactions – from in vivo functionality to pharmaceutics and food science

In-lab SEC-SAXS for structural investigation of complex samples

Microgels

Vesicles

High quality SAXS data in the lab

Measurements previously only possible at synchrotron facilities can now be done in the lab.

With the BioXolver you get:

• Excellent data suitable for modelling and publications.
• Precision determination of key structural parameters.
• Structural information of proteins in solutions on a daily basis as complementary information to other characterization techniques.

Due to weak scattering from biological samples, high quality SAXS measurements require an instrument optimized for maximum X-ray intensity and lowest possible background noise. The BioXolver offers a unique combination of both capabilities.

High throughput measurements and results

Full automation from sample to final results on up to 192 samples.

With the BioXolver standard well plates can be loaded & measured automatically using in-line pipetting robot & automated software suite for high user benefits.

• More samples can be run
• Short sample-to-sample cycle time
• Reproducibility of your measurement is insured

Low sample volume consumption

Down to 5μL. The lowest sample consumption on the market.

High precision positioning of small sample volumes is ensured by the conjunction of an in-line pipetting robot and the BioCUBE, an advanced capillary flow cell, coupled to machine vision for sample monitoring.

Such low total consumption per sample condition or concentration is highly beneficial for biostructural research.

• Less precious sample is needed.
• More experiments can be performed from the same preparation volume.

In-line SEC and UV-Vis combined with SAXS – also in the lab!

Obtain the structure of small and large protein species through in-line gel filtration

Sample size separation using size-exclusion chromatography in combination with the BioXolver can not only be used to ensure clean monodisperse samples, but also facilitates research on structurally heterogeneous & aggregation-prone multi-domain proteins as well as heterogeneous protein complexes in dynamic equilibria.