본 제품은 사람의 nasopharyngeal swab 검체, nasopharyngeal aspirate 검체, bronchoalbeolar lavage (BAL) 검체에서 추출한 genomic DNA로 Real-Time PCR 방식을 이용하여 13종 폐렴균을 정성적으로 동시 또는 단독으로 검출하는 키트입니다.
제품사양
DiaPlexQ™ PneumoPatho 13 Detection Kit
Detection target
Set I : M. pneumoniae (MP) K. pneumoniae (KP) L. pneumophila (LP)
Set III : P. aeruginosa (PA) M. catarrhalis (MC) B. pertussis (BP)
Set II : S. pneumoniae (SP) S. aureus (SA) C. pneumoniae (CP)
Set IV : H. influenzae (HI) A. baumannii (AB) M. tuberculosis/ M. avium (TB/AV)
ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) CFX96™ Real-Time PCR System (Bio-Rad)
PCR running time
~ 2 hrs
제품특징
- HotStart PCR system : Ultra high specific and sensitive result
- UDG system : No carryover contamination
- Multiplex PCR system : Multiple targets in a single reaction
- Reliable system : Automatic PCR control
- Positive control included
- Easy-to-use master mix
실험데이터
인용&논문
Micheal J McConnell et al.
Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment. Journal of Clinical Microbiology p. 1412-1414.
Ko WC, Paterson DL, Sagnimeni AJ,
et al. Community-acquired Klebsiella pneumonia bacteremia: global differences in clinical patterns. Emerg Infect Dis 2002; 8:160-6.
Gierczynski R, Jagielski M, Rastswicki W, Klauzewski S (2007)
Multiplex-PCR assay for idenrification of Klebsiella pneumonia isolates carrying the chain loci for K1 and K2 capsule biosynthesis. Pol J Microbiol 56: 153-156.
주문정보 (Ordering information)
Technology
Cat. No.
Product
Contents
Real-Time PCR
SQD81-K020 (20 reaction)
DiaPlexQ ™ PneumoPatho 13 Detection Kit
2X Multiplex Real-Time PCR Smart mix (with UDG) (PneumoPatho 13) Primer & Probe Mixture I (PneumoPatho 13) Primer & Probe Mixture II (PneumoPatho 13) Primer & Probe Mixture III (PneumoPatho 13) Primer & Probe Mixture IV (PneumoPatho 13) Control Template I (PneumoPatho 13) Control Template II (PneumoPatho 13) Control Template III (PneumoPatho 13) Control Template IV (PneumoPatho 13) Nuclease free Water