Chat with us, powered by LiveChat DiaPlexQ™ Avellino Corneal Dystrophy (ACD) Real-Time PCR Genotyping Kit (Corneal Dystrophy) | SOLGENT - Komachine
Product Name DiaPlexQ™ Avellino Corneal Dystrophy (ACD) Real-Time PCR Genotyping Kit (Corneal Dystrophy)
Model
Series Molecular Diagnostic (Human Gentrifyping)
Catalog
Product Description

DiaPlexQ™ Avellino Corneal Dystrophy (ACD) Real-Time PCR Genotyping Kit

Real-Time PCR based assay system for the genotyping of the ACD gene SNP related to avellino corneal dystrophy

CE-IVD


ACD is a hereditary disease and one of the corneal dystrophies involving the formation of corneal opacities on different Layers of the corneal stroma, which leads to significant impairment of the corneal transparency and refraction. ACD is caused by the formation and deposition of abnormal hyaline protein which is generated by the replacement of histidine from arginine due to the mutation of cordon 124 (exon 4) in the βigh 3 gene. The deposited abnormal hyaline protein is usually known as kerato-epithelin and forms a granular, lattice precipitate causing visual impairment and might lead to blindness if it remains undiagnosed. Genetic mutation is highly accelerated after laser eye surgery in those people who have ACD, causing a worsening in vision rather than improvement after surgery. The awareness of ACD has developed across the world, with some opticians now refusing access to LASIK in patients with ACD due to the harmful effects the procedure has on patients.




Specification


DiaPlexQ ™ Avellino Corneal Dystrophy (ACD) Real-Time PCR Genotyping Kit
Detection targetSNP of R124 (βigh3 coding gene codon 124, exon4)
RegistrationCE-IVD
Detection technologyReal-Time PCR
Specimen typeBlood, Buccal epithelial cell, Hair (root)
Compatible instruments*ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
CFX96™ Real-Time PCR System (Bio-Rad)
PCR running time~ 1hr 30 min




Features

- HotStart PCR system : Ultra high specific and sensitive result

- UDG system : No carryover contamination

- Multiplex PCR : Multiple targets in a single reaction

- Reliable system : Automatic Internal control ( DiaPlex C™)

- Positive control included

- Easy-to-use master mix

- CE certification





Experimental


ColorGenotyping
RedACD G/G type
BlueACD A/A type
GreenACD G/A type
● BlackNon-Template Control






Citation&Papers

Kocak-Atlintas AG, Kocak-Midillioglu I, Akarsu AN, Duman S.

βigh gene analysis in the different diagnosis of corneal dystrophies. Cornea 2001; 20: 64-8.

Klintworth GK.

Advances in the molecular genetics of corneal dystrophies. Am J phthalmol 1999; 128: 747-54.

Konishi M, Mashima Y, Nakamura Y, et al.

Granular-lattice (Avellino) corneal dystrophy in Japanese patients. Cornea 1997; 16: 635-8.





Ordering information

TechnologyCat. No.ProductContents



Real-Time PCR
SQH26-K020
(20 reaction)


DiaPlexQ ™ Avellino Corneal Dystrophy (ACD)
Real-Time PCR Genotyping Kit
2X Multiplex Real-Time PCR Smart mix (ACD)
Primer & Probe Mixture (ACD)
A/A type Control DNA (ACD)
G/A type Control DNA (ACD)
G/G type Control DNA (ACD)
Nuclease free Water
SQH26-K100
(100 reaction)




Products of SOLGENT
Research Reagents (PCR)
Molecular Diagnostic (Pathogen Detection)