DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
DiaPlexC™ Influenza Virus A/B Detection Kit
Multiplex PCR based assay system for simultaneous detection of influenza virus A and B
CE-IVD
Influenza is a family of RNA enveloped viruses that affects mammals and birds. Among three types of influenza viruses A, B and C. Influenza A is the most important in human infection. Influenza is a huge public health concern that occur in Seasonal epidemic and has caused pandemic level infections. There are three types of influenza viruses; A, B and C. In which specificity is conferred by internal nucleoprotein and matrix protein antigens. Influenza A and B are the major Causative agents of human acute respiratory disease worldwide, while Influenza virus C occurs much less frequently than A and B, That is why only influenza A and B are included in seasonal influenza vaccines.
Specification
| DiaPlexC™ Influenza Virus A/B Detection kit | ||
|---|---|---|
| Detection target | Influenza Virus A , Influenza Virus B | |
| Registration | CE-IVD | |
| Detection technology | Conventional (End-point) OneStep Multiplex RT-PCR | |
| Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum | |
| Analytical sensitivity | 10 - 10² copies | |
| Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended | |
| PCR running time | ~ 2 hrs 30 min | |
Features
- Simple & Rapid detection system : OneStep Multiplex qRT-PCR (or RT-PCR) based detection
- HotStart PCR system : Ultra high specific and sensitive result
- Reliable system : Automatic PCR control & Internal control
- Positive control included
- CE certification
Experimental
| Sample type | FAM | Cy5 | Texas Red / Cal Red 610 | Result |
|---|---|---|---|---|
| Positive Control | + | + | + | Valid |
| Negative Contro | - | - | + | Valid |
| NTC (Non-Template Control) | - | - | + | Valid |
| Sample case 1 | + | - | +/- | Influenza A |
| Sample case 2 | - | + | +/- | Influenza B |
| Sample case 3 | + | + | +/- | Influenza A&B (Co-infection) |
| Sample case 4 | - | - | + | Negative |
| Sample case 5 | - | - | - | Required re-experiment |
| Lane | Interpretation (detection) |
|---|---|
| 1 | Negative |
| 2 | Influenza Virus A |
| 3 | Influenza Virus B |
| 4 | NTC |
| 5 | Required re-experiment |
Citation&Papers
Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al. J Virol. 2002;79:2814-2822 | Lamb RA, Krug R. Field Virology, third ed. Lippincott-Raven Philadelphia. 1996; 1353-1395. | Ha Y, Stevens, DJ, Wiley DC. The EMBO Journal. 2002;21;865-875. | Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al. Virology. 2004;287-296. |
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
Real-Time PCR | SQD42-K020 (20 reaction) | DiaPlexQ ™ Influenza Virus A/B Detection kit | OneStep qRT-PCR Enzyme mix (Inf A/B) 2X OneStep qRT-PCR Buffer (Inf A/B) Primer & Probe Mixture (Inf A/B) Control Template (Inf A/B) RNase free Water |
| SQD42-K100 (100 reaction) | |||
Conventional (End-point) PCR | SMD42-K020 (20 reaction) | DiaPlexC ™ Influenza Virus A/B Detection kit | OneStep RT-PCR Enzyme mix (Inf A/B) 2X OneStep RT-PCR Buffer (Inf A/B) Primer Mixture (Inf A/B) Standard Marker (Inf A/B) Control Template (Inf A/B) RNase free Water |
| SMD42-K100 (100 reaction) |
