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Product Name DiaPlexQ™ Influenza Virus A/B Detection Kit (Respiratory Disease)
Model
Series Molecular Diagnostic (Pathogen Detection)
Catalog
Product Description

DiaPlexQ™ Influenza Virus A/B Detection Kit

Real-Time OneStep RT-PCR based assay system for simultaneous detection of influenza virus A and B

CE-IVD


Influenza is a family of RNA enveloped viruses that affects mammals and birds. Among three types of influenza viruses A, B and C. Influenza A is the most important in human infection. Influenza is a huge public health concern that occur in Seasonal epidemic and has caused pandemic level infections. There are three types of influenza viruses; A, B and C. In which specificity is conferred by internal nucleoprotein and matrix protein antigens. Influenza A and B are the major Causative agents of human acute respiratory disease worldwide, while Influenza virus C occurs much less frequently than A and B, That is why only influenza A and B are included in seasonal influenza vaccines.




Specification


DiaPlexQ™
Influenza Virus A/B Detection kit

Detection targetInfluenza Virus A , Influenza Virus B
RegistrationCE-IVD
Detection technologyReal-Time OneStep RT-PCR
Specimen typeNasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum
Analytical sensitivity10 - 10² copies
Compatible instruments*ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
CFX96™ Real-Time PCR System (BioRad)

PCR running time~ 3 hrs




Features

- Simple & Rapid detection system : OneStep Multiplex qRT-PCR (or RT-PCR) based detection

- HotStart PCR system : Ultra high specific and sensitive result

- Reliable system : Automatic PCR control & Internal control ( DiaPlexQ ™ : only PCR control)

- Positive control included

- CE certification




Experimental


Sample typeFAMCy5Texas Red / Cal Red 610Result
Positive Control+++Valid
Negative Contro--+Valid
NTC (Non-Template Control)--+Valid
Sample case 1+-+/-Influenza A
Sample case 2-++/-Influenza B
Sample case 3+++/-Influenza A&B (Co-infection)
Sample case 4--+Negative
Sample case 5---Required re-experiment



LaneInterpretation (detection)
1Negative
2Influenza Virus A
3Influenza Virus B
4NTC
5Required re-experiment



Citation&Papers

Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al.

J Virol. 2002;79:2814-2822

Lamb RA, Krug R. Field Virology, third ed.

Lippincott-Raven Philadelphia. 1996; 1353-1395.

Ha Y, Stevens, DJ, Wiley DC.

The EMBO Journal. 2002;21;865-875.

Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al.

Virology. 2004;287-296.





Ordering information

TechnologyCat. No.ProductContents


Real-Time PCR
SQD42-K020 (20 reaction)
DiaPlexQ ™ Influenza Virus A/B Detection kit
OneStep qRT-PCR Enzyme mix (Inf A/B)
2X OneStep qRT-PCR Buffer (Inf A/B)
Primer & Probe Mixture (Inf A/B)
Control Template (Inf A/B)
RNase free Water
SQD42-K100 (100 reaction)



Conventional (End-point) PCR
SMD42-K020 (20 reaction)

DiaPlexC ™ Influenza Virus A/B Detection kit
OneStep RT-PCR Enzyme mix (Inf A/B)
2X OneStep RT-PCR Buffer (Inf A/B)
Primer Mixture (Inf A/B)
Standard Marker (Inf A/B)
Control Template (Inf A/B)
RNase free Water
SMD42-K100 (100 reaction)


Products of SOLGENT
Molecular Diagnostic (Pathogen Detection)
Research Reagents (PCR)