DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
DiaPlexC™ M.Avium/M.Intracellulare Detection Kit
Multiplex PCR based assay system for simultaneous detection of M. avium and M. intracellulare among NTM complex
Mycobacterium avium and Mycobacterium intracellulare complex (MAC) is the atypical mycobacterium most commonly associated with human disease. MAC is primarily a pulmonary pathogen that affects individuals whose immune systems have been compromised (e.g. from AIDS, hairy cell leukemia, immunosuppressive chemotherapy). In this clinical setting, MAC has been associated with osteomyelitis, tenosynovitis and synovitis. MAC comprises two genetically distinct but Difficult to discriminate species. M. avium predominates (87 % - 98 %) in AIDS patients with no anti-retroviral therapy and M. intracellulare is more frequent among non-AIDS patients.
Specification
| DiaPlexC™ M.Avium/M.Intracellulare Detection Kit | |
|---|---|
| Detection target | Mycobacterium avium , Mycobacterium intracellulare |
| Registration | CE-IVD |
| Detection technology | Conventional (End-point) Multiplex PCR |
| Specimen type | Sputum, Bronchial lavage, Lung biopsy, Gastric lavage, Urine, Body fluid, Blood, Tissue, Pus, Stool |
| Analytical sensitivity | 10³ copies |
| Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended |
| PCR running time | ~ 2 hrs |
Features
- HotStart PCR system : ultra high specific & sensitive result
- DnaFree™ system : No host genomic DNA contamination
- UDG system : No carryover contamination
- Multiplex PCR system : Multiple targets in a single reaction
- Reliable system : Automatic PCR control
- Positive control included
- Easy-to-use master mix
- CE certification
Experimental
| Lane | Interpretation (detection) |
|---|---|
| 1 | Negative |
| 2 | M. intracellulare |
| 3 | M. avium |
| 4 | M. intracellulare , M. avium (Co-infection) |
| 5 | NTC |
| 6 | Required re-experiment |
Citation&Papers
The NTM handbook: A Guide for Patients with Non-Tuberculous Mycobacterial Infections Including MAC. | Horsburgh CR Jr. Epidemiology of Mycobacterium avium complex. In: Korvick JA, Benson CA, eds. Mycobacterium Avium Complex Infection: Progress in Research and Treatment. New York, NY: Marcel Dekker; 1996:1-22. | Tortoli E. Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev. 2003; 16:319-354. | Griffith, David, E.; Aksamit Timothy; & A. Brown-Elliott, Barbara et al. (2007). American Thoracic Society Guidelines: Diagnosis, Treatment and Prevention of Non-tuberculous Mycobacterial Diseases. AM. J. Respiratory and Critical Care Medicine, Vol. 175, pp. 367-417. | Grange, J.M. (2007). “Environmental mycobacterial”. In Greenwood, David; Slack, Richard; peitherer, John; & Barer, Mike (Eds.), Medical Microbiology (17th Ed.), pp. 221-227. Elsevier. ISBN 978-0-443-10209-7. Evans AJ, Crisp AJ, Hubbard RB, Colville A, Evans SA, Johnston IDA. Pulmonary Mycobacterium kansasii infection: comparision of radiological appearances with pulmonary tuberculosis. Thorax. 1996;51:1243-1247. |
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
| Conventional (End-point) PCR | SMD23-K020 (20 reaction) | DiaPlexC ™ M.Avium/M.Intracellulare Detection Kit | 2X Multiplex PCR Smart mix (with UDG) (M.Av/M.Int) Primer Mixture (M.Avium/M.Intra) Standard Marker (M.Avium/M.Intra) Control Template (M.Avium/M.Intra) Nuclease free Water |
| SMD23-K100 (100 reaction) |
