DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
DiaPlexC™ MTB/M.Bovis Detection Kit
Multiplex PCR based assay system for simultaneous detection of MTB and M. bovis among MTC complex
CE-IVD
Mycobacterium tuberculosis (TB) is one of the most ubiquitously found dominant infectious agents around the world especially in developing countries that cause tuberculosis for almost 8 million and the death of 2 million people each year. Though there is a prevention method, the BCG (Bacillus Calmette-Guerin) TB vaccination, its disadvantage is that it cannot protect after 5 years from immunization and there is also possibility of diagnostic error for actual TB because there is a high chance of a false-positive result in the tuberculin test.
Specification
| DiaPlexC™ MTB/M. Bovis Detection Kit | |
|---|---|
| Detection target | Mycobacterium tuberculosis (MTB), Mycobacterium bovis (M. bovis) |
| Registration | CE-IVD |
| Detection technology | Conventional (End-point) Multiplex PCR |
| Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum |
| Analytical sensitivity | 10² copies |
| Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended |
| PCR running time | ~ 2 hrs |
Features
- HotStart PCR system : ultra high specific and sensitive result
- DnaFree™ system : No host genomic DNA contamination
- UDG system : No carryover contamination
- Multiplex PCR system : Multiple targets in a single reaction
- Reliable system : Automatic PCR control
- Positive control included
- Easy-to-use master mix
- CE certification
Experimental
| Lane | Interpretation (detection) |
|---|---|
| 1 | Negative (or NTC) |
| 2 | MTB |
| 3 | M. bovis |
| 4 | MTB, M. bovis (Co-infection) |
| 5 | Required re-experiment |
Citation&Papers
“Mycobacterium tuberculosis” Sanger Institute. 2007-03-29 http://www.sanger.ac.uk/projects/M_tuberculosis/. Retrieved 2008-11-16 | Frothingham R, Meeker-Meeker-O’Connell WA. (1998). “Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats.”. Microbiology 144 (Pt 5): 1189-96. | Teo, SS ; Shingadia, DV (2006 Jun). “Does BCG have a role in tuberculosis control and prevention in the United Kingdom?”. Archives of Disease in Childhood 91 (6) : 529-31. |
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
| Conventional (End-point) PCR | SMD22-K020 (20 reaction) | DiaPlexC ™ MTB/M. Bovis Detection Kit | 2X Multiplex PCR Smart mix (with UDG) (MTB/M. Bovis) Primer Mixture (MTB/M. Bovis) Standard Marker (MTB/M. Bovis) Control Template (MTB/M. Bovis) Nuclease free Water |
| SMD22-K100 (100 reaction) |
