DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
DiaPlexQ™ MTC/NTM Detection Kit
Real-Time PCR based assay system for simultaneous detection of MTC and NTM complex
CE-IVD
M y cobacterium tuberculosis (MTB) is a pathogenic bacteria species and the causative agent in most cases of tuberculosis. Along with MTB, 5 other species, which we call MTC are regarded as common infectious agents causing tuberculosis. That is why it is necessary to detect MTC, along with MTB. Although NTM is a group of non-tuberculosis related mycobacteria, it usually causes lung infections that mimic tuberculosis resulting in a potential misdiagnosis. Thus, the identification of a single infection or co-infection of MTC and NTM is required for appropriate treatment.
Specification
| DiaPlexQ™ MTC/NTM Detection Kit | DiaPlexQ™ MTC/NTM Detection Kit ver 3.0 | DiaPlexQ™ MTC/NTM Detection Kit ver 4.0 | |
|---|---|---|---|
| Detection target | MTC (2 species): IS6110 NTM (13 species): 16S rRNA | MTC (2 species): IS6110, MPB64 NTM (15 species): 16S rRNA | MTC (2 species): IS6110, MPB64 NTM (6 species): 16S rRNA |
| Registration | KFDA, CE-IVD | CE-IVD | CE-IVD |
| Detection technology | Real-Time PCR | ||
| Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL) | ||
| Analytical sensitivity | 10 copies | ||
| Compatible instruments* | ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) CFX96™ Real-Time PCR System (Bio-Rad) | ||
| PCR running time | ~ 1 hr 20 min | ||
Features
- HotStart PCR system : Ultra high specific and sensitive result
- DnaFree™ system : No host genomic DNA contamination ( DiaPlexC ™)
- UDG system : No carryover contamination
- Multiplex PCR system : Multiple targets in a single reaction
- Reliable system : Automatic PCR control
- Positive control included
- DNA extraction solution included (Cat. No. SQD21-K100)
- Easy-to-use master mix
- CE certification
Experimental
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| Sample type | FAM | HEX | Cy5 | Result |
|---|---|---|---|---|
| Positive Control | + | + | + | Valid |
| Negative Control | - | - | + | Valid |
| NTC(Non-Template Control) | - | - | + | Valid |
| Sample case 1 | + | + | +/- | MTC, NTM (Co-infection) |
| Sample case 2 | + | - | +/- | MTC |
| Sample case 3 | - | + | +/- | NTM |
| Sample case 4 | - | - | + | Negative |
| Sample case 5 | - | - | - | Required re-experiment |
Citation&Papers
Korean J Clin microbial, Seong Deok Lee, Hye Young Lee, Hyun Chul Kim, Soo Young Kim, Mycobacterium tuberculosis and Non-Tuberculous Mycobacteria by PCR Assay. | Ryan KJ, Ray CG (Editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw-Hill. Clinical Characteristics of Non-Tuberculous Mycobacterial Pulmonary Disease in a National Tuberculosis Hospital. | Sun-Pil Choi, M.D., Bong-Keun Lee M.D.1, Jin-Hong Min, M.D., Jin-Hee Kim, M.D. Pathogenic Classification and Clinical Charateristics of Non-Tuberculous Mycobacterial Pulmonary Disease in a National Tuberculosis Hospital. |
Ordering information
| Technology | Cat. No. | Product |
|---|---|---|
| Real-time PCR | SQD21-K100 | DiaPlexQ™ MTC/NTM Detection Kit (w/Ext.) |
| SQD20-K100 | DiaPlexQ™ MTC/NTM Detection Kit | |
| SQD26-K100 | DiaPlexQ™ MTC/NTM Detection Kit (w/Ext.)- Ver.3.0 | |
| SQD25-K100 | DiaPlexQ™ MTC/NTM Detection Kit- Ver.3.0 | |
| SQD28-K100 | DiaPlexQ™ MTC/NTM Detection Kit (w/Ext.)- Ver.4.0 | |
| SQD27-K100 | DiaPlexQ™ MTC/NTM Detection Kit- Ver.4.0 |
