Ligation enzyme Connection of cohesive end / blunt end PCR product
SolgTM T4 DNA Ligase is a ligation enzyme and it is designed to connect and combine 5’-phosphate and 3’-OH phosphodiester by using of ATP as a cofactor which is laid in a parallel. This product is useful to connect both of cohesive end and blunt end PCR product. SolgTM T4 DNA Ligase is used to connect nick which is formed in a single strand.
Specification
Source
Bacteriophage T4 (Recombinant)
Ligation temp. & time
16 ℃ for 2 hr or 20 ~ 25 ℃ for 1 hr
Inactivation
70 ℃ for 10 min
Application
- Cohesive or Blunt-end ligation, Cloning of r estriction fragments, Joining linkers and adapters to blunt-ended DNA, Repairing nicks (in duplex DNA, RNA or DNA-RNA h ybrids)
Format
Product Image
Contents
Storage & Expiry Date
SolGent™ T4 DNA Ligase 10X Ligase Buffer
2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)