DiaStar™ Quick DNA Ligase is used to form a phosphodiester bond by joining the DNA 5’-phosphate and 3’-OH with the help of cofactor ATP. It’s ligation reaction time is 5 minutes at room temperature and can be applied for both cohesive and blunt end type of DNA strands. In addition, double strand formed by the process is also used for the connection.
Specification
Source
Bacteriophage T4 (Recombinant)
Ligation temp. & time
20 ~ 25 ℃ for 5 min
Inactivation
Not necessary
Application
- Cloning into vectors, Linker ligation, Library construction, Re-circulization of linear DNA
Format
Product image
Contents
Storage & Expiry date
DiaStar™ Quick DNA Ligase
2X Ligase Buffer
2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)