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SOLGENT
[SCA] SEQ-TempliGen™ WGA Kit (Isothermal Amplification)
Model
Series
Research Reagents (PCR)
Transaction Process
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Description
[SCA] SEQ-TempliGen WGA™ Kit
Whole genome amplification Kit
SEQ-TempliGen™ WGA Kit is a product for isothermal amplification of genomic DNA by Phi29 DNA polymerase. It enables DNA amplification rapidly and accurately using MDA (Multiple Displacement Amplification) mechanism within 4 hrs with small amount of sample by optimized buffer system and innovative DNA polymerase.
Specification
| Source | Bacillus subtilis phage Phi29 | Amplification fold | 10³ ~ 10⁹ |
| 5’→3’exonuclease activity | No | Error rate | 1 bp error / 10⁶ - 10⁷ bp |
| 3’→5’Exonuclease activity | Yes | Sample (Template) | Purified genomic DNA |
| Strand displacement activity | Yes | Minimum Concentration of Template | 10ng≤ |
| Reaction temperature | 30 ℃ | Amplification Time | ~2 hours |
Application
- Unbiased amplification of whole genome , Pr eservation, Next generation sequencing, SNP / SSR genot yping analysis, PCR and Real-Time PCR, High fidelit y replication at moderate temperatures
Format
| Product Image | Contents | Storage & Expiry Date |
|---|---|---|
  | 8X WGA denaturation buffer (8X DB) 10X WGA neutralization buffer (10X NB) WGA sample buffer (SB) WGA reaction buffer (RB) WGA enzyme mixture (EM) | 1 year 6 months (In case of Store at -20 ℃ ± 5 ℃) |
Ordering information
| Cat. No. | Product | Size | Price(won) |
|---|---|---|---|
| SCA80-K025 | SEQ-TempliGen™ WGA Kit | 25 reaction | - |
| SCA80-K100 | SEQ-TempliGen™ WGA Kit | 100 reaction | - |