Search

SOLGENT

SOLGENT

SOLGENT

SOLGENT

HomeManufacturersSOLGENT
1/1
SOLGENT

SOLGENT

[DR] DiaStar™ OneStep RT-PCR Series (cDNA Synthesis)

Model

Series

Research Reagents (PCR)


Transaction Process

Please contact the manufacturer/supplier.

Payment

Contact the manufacturer/supplier.

Delivery

Contact the manufacturer/supplier.

Shipment

Contact the manufacturer/supplier.

Origin

Contact the manufacturer/supplier.


Description

[DR] DiaStar™ OneStep RT-PCR Series

For cDNA synthesis & PCR amplification at ‘One Step’ from RNA template


DiaStar™ OneStep RT-PCR Kit is a product for easy reverse transcription and PCR at “One Step” with optimal recombinant of DiaStarTM Reverse Transcriptase and Solg™ HotStart h-Taq DNA Polymerase mixture. This kit has dual advantages of high efficiency for cDNA synthesis and high specificity of hot start polymerase to set up of optimal PCR condition easily and conveniently



Specification

Reverse transcriptionSourceM-MLV (Recombinant)RNase H activityRNase H-
cDNA length~ 14 kb
Reaction temperature42 – 55 ℃Applicable PrimersGene-specific primer
PCRSourceThermus aquaticus (Solg™ h-Taq)Hot start activityYes (Chemical-mediated)
Amplicon size~ 1 kb3’A or Blunt ends3’A



Application

One-Step Multiplex RT-PCR, Gene expression analysis, RNA virus research, TA cloning




Citation&Papers

- Chung EY, Kim BH,

   et al., Resveratrol down-regulates interferon-γ-inducible inflammatory genes in macrophages: molecular mechanism via decreased STAT-1 

   activation, JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2010




Format

FormatProduct ImageContentsStorage & Expiry Date
Standard

DiaStar™ OneStep RT-PCR Enzyme Mix
5X OneStep RT-PCR Buffer
each 10 mM dNTP Mix
5X Band Doctor™
RNase-free water
1 year (In case of Store at -20 ℃ ± 5 ℃)
Smart mix

2X OneStep RT-PCR Smart mix1 year (In case of Store at -20 ℃ ± 5 ℃)
Pre-Mix

2X OneStep RT-PCR Pre-Mix1 year (In case of Store at -20 ℃ ± 5 ℃)




Ordering information

FormatCat. No.ProductSizePrice (won)
StandardDR61-K050DiaStar™ OneStep RT-PCR Kit50 reaction-
DR61-K100DiaStar™ OneStep RT-PCR Kit100 reaction-
Smart mixDR31-M10hDiaStar™ 2X OneStep RT-PCR Smart mix, with dye²1 ㎖
(66 reaction)¹
-
DR31-M50hDiaStar™ 2X OneStep RT-PCR Smart mix, with dye1 ㎖ x 5 ea-
Pre-MixDR31-P096DiaStar™ 2X OneStep RT-PCR Pre-Mix, with dye, total volume 30 ㎕ reaction96 tube
(8 strip x 12 ea)
-
Single itemDR65-B50hDiaStar™ 5X OneStep RT-PCR Reaction Buffer1 ㎖ x 5 ea
(833 reaction)
-



Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

ⓒ2024 Komachine Inc. All rights reserved.