DiaPlexQ™ Novel Coronavirus (2019-nCoV) Detection Kit, Covid19, Corona virus
[SEF] Solg™ EF-Taq Polymerase
PCR enzyme for long fragment amplification
Long-range PCR
High productivity / Low error rate
Amplicon size : ~ 20 kb
Solg™ EF-Taq polymerase is chemical-mediated HotStart PCR enzyme, which enables multiplex and allele specific PCR for diagnostics and genotyping through initial heat activation during PCR cycling. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.
Specification
| Source | Pyrococcus furiosus & Thermus aquaticus (Recombinant) | Amplicon size | ~ 20 kb |
| Hot start activity | No | ||
| 5’→ 3’ exonuclease activity | Yes | 3’A or Blunt ends | 3’A |
| 3’→ 5’ exonuclease activit y | Yes (weak) | Error rate | 5-6 bp error / 10⁶ bp |
Application
- Long-range PCR, High productivity PCR, Inverse PCR, TA cloning
Citation&Papers
Li CS, Zhang QG, et al., Interleukin-27 polymorphisms are associated with inflammatory bowel diseases in a Korean population, Journal of Gastroenterology and Hepatology , 24 : 1692-1696, 2009 | Fu XT, Lin H, et al., Purification and characterization of a novel β-agarase, AgaA34, from Agarivorans albus YKW-34, Applied Microbiology and Biotechnology , 78 : 265-73, 2008 |
Format
| Format | Product Image | Contents | Storage & Expiry Date |
|---|---|---|---|
| Standard |   | Solg ™ EF-Taq DNA Polymerase (2.5 U/㎕) 10X EF-Taq Reaction Buffer (25 mM MgCl₂mixed) 10 mM dNTP Mix 5X Band Doctor™ | 2 years 3 months (In case of Store at -20 ℃ ± 5 ℃) |
| Smart mix |   | 2X EF-Taq PCR Smar t mix | 1 year 6 months (In case of Store at -20 ℃ ± 5 ℃) |
| Pre-Mix |   | 2X EF-Taq PCR Pre-Mix | 1 year 6 months (In case of Store at -20 ℃ ± 5 ℃) |
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Standard | SEF16-R250 | Solg ™ EF-Taq DNA Polymerase | 250 U (200 reaction) | - |
| SEF16-R500 | Solg ™ EF-Taq DNA Polymerase | 500 U | - | |
| SEF16-R25h | Solg ™ EF-Taq DNA Polymerase | 2,500 U (500 U x 5 ea) | - | |
| SEF16-R50h | Solg ™ EF-Taq DNA Polymerase | 5,000 U (500 U x 10 ea) | - | |
| Smart mix | SEF01-M50h | Solg ™ 2X EF-Taq PCR Smar t mix 1, with dy e | 1㎖ x 5 ea (200 reaction) | - |
| SEF02-M50h | Solg ™ 2X EF-Taq PCR Smar t mix 2 (0.5X B and Doctor), with dye | 1㎖ x 5 ea | - | |
| Pre-Mix | SEF02-P096 | Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye, total volume 30㎕ reaction | 96 tube (8 strip x 12 ea) | - |
| SEF02-P288 | Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye, total volume 30㎕ reaction | 288 tube (8 strip x 36 ea) | - | |
| SEF02-P576 | Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye, total volume 30㎕ reaction | 576 tube (8 strip x 72 ea) | - | |
| Single item | SEF22-B50h | DiaStar™ 10X Taq Reaction Buf fer | 1 ㎖ x 5 ea (1,000 reaction) | - |
