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[DD] DiaStar™ Fh-Taq DNA Polymerase (DNA-free)
99.9% removal of host DNA contamination in the enzyme, No false positive
Host DNA contamination free system
Chemical-mediated Hot start enzyme
Ultra-high specificity
Amplicon size : ~ 1 kb
Diastar™ Fh-Taq DNA Polymerase (DNA-free) is high purity Taq DNA polymerase which is applied to DNA-free and HotStart system. So it is suitable screening to variety pathogenic bacterium without false positive amplification. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.
Specification
| Source | Thermus aquaticus (Recombinant) | Amplicon size | ~ 1 kb |
| Hot start activity | Yes(Chemical-mediated) | ||
| 5’→ 3’ exonuclease activity | Yes | 3’A or Blunt ends | 3’A |
| 3’→ 5’ exonuclease activit y | No | Error rate | 12-13 bp error / 10⁶ bp |
Application
- 16s rRNA identification, Metagenome analysis, Molecular diagnosis, Allele specific PCR Highly specific amplification of genomic and cDNA tar gets, SNP detection, TA cloning
Format
| Format | Product Image | Contents | Storage & Expiry Date |
|---|---|---|---|
| Standard |   | DiaStar™ Fh-Taq DNA Polymerase (DNA-free) (2.5 U/㎕) 10X Fh-Taq Reaction Buffer (25 mM MgCl₂mixed) 10 mM dNTP Mix 5X Band Doctor™ | 2 years 3 months (In case of Store at -20 ℃ ± 5 ℃) |
Ordering information
| Format | Cat. No. | Product | Size | Price(won) |
|---|---|---|---|---|
| Standard | DD36-R250 | DiaStar™ Fh-Taq DNA Polymerase (DNA-free) | 250 U (200 reaction) | - |
| DD36-R10h | DiaStar™ Fh-Taq DNA Polymerase (DNA-free) | 1,000 U (250 U x 4 ea) | - | |
| DD68-R250 | DiaStar™ Fh-Taq DNA Polymerase (DNA-free) with 10 mM dNTP(U) Mix | 250 U | - | |
| Single item | DD22-B50h | DiaStar™ 10X Fh-Taq Reaction Buffer | 1 ㎖ x 5 ea (1,000 reaction) | - |
