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Product Name DiaPlexQ™ Apolipoprotein E (ApoE) Genotyping Kit (Alzheimer's Disease)
Model
Series Molecular Diagnostic (Human Gentrifyping)
Catalog
Product Description

DiaPlexQ™Apolipoprotein E (ApoE) Genotyping Kit

Real-Time PCR based assay system for the genotyping of the ApoE gene SNP related to alzheimer’s disease

CE-IVD


Apolipoprotein E (ApoE) consists of three isoforms: E2, E3 and E4. These three isoforms make six genotypes, (homozygous genotypes: E2/E2, E3/E3 E4/E4 and heterozygous genotypes: E2/E3, E2/E4, E3/E4). Isoform E2 is usually known as a protective isoform whereas isoform E4 is generally responsible for diseases. The predominant abnormalities are due to a single nucleotide polymorphism (SNP) of codon 112 (Cys/Arg) and codon 158 (Arg/Cys). The polymorphism has functional effects on the lipoprotein metabolism. ApoE is the primary ligand for two receptors, LDL receptors found in the liver and other tissues and ApoE specific receptors found in the liver. The co-ordinate interaction of these lipoprotein complexes with their receptors forms the basis for the metabolic regulation of cholesterol. ApoE has been found to be associated with elevated cholesterol levels or lipid derangements that lead to various clinical problems like coronary heart diseases, strokes, periphery artery diseases, and diabetes mellitus. In addition to the genotype-phenotype associations with the cardiovascular disease, the alleles and isoforms of ApoE have been related to dementias, most commonly Alzheimer’s disease. Determining the genotype of ApoE is thus considered to be an important test for patients with the disease, as well as a precautionary test for healthy people.





Specification


DiaPlexQ™ ApoE Genotyping Kit
Detection targetApoE genotype (Mutation T112C and T158C of ApoE gene) E2/E2, E3/E3, E4/E4, E2/E3, E2/E4, E3/E4
RegistrationCE-IVD
Detection technologyReal-Time PCR
Specimen typeBlood
Compatible instruments*ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
CFX96™ Real-Time PCR System (Bio-Rad)

PCR running time~ 1 hr 30 min




Features

- HotStart PCR system : Ultra high specific and sensitive result

- UDG system : No carryover contamination ( DiaPlexQ™)

- Multiplex PCR : Multiple targets in a single reaction

- Reliable system : Automatic Internal control ( DiaPlexC™)

- Positive control included

- Easy-to-use master mix

- CE certification





Experimental




ApoE genotypeSNP112 site158 site
E2/E2TT/TT T/T type● T/T type
E2/E3TT/TC● T/T type T/C type
E2/E4TC/TC T/C type T/C type
E3/E3TT/CC● T/T type C/C type E4
E3/E4TC/CCT/C type● C/C type E4
E4/E4CC/CC● C/C type E4● C/C type E4
● Non-Template Control








Citation&Papers

Lepara O, Valjevac A, Alajbegovic A, Zaciragic A, Nakas-Icindic E (August 2009).

Decreased serum lipids in patients with probable Alzheimer’s disease”. Bosn J Basic Med Sci 9 (3): 215-20.

Golomb BA, Evans MA (2008).

“Statin adverse effects : a review of the literature and evidence for a mitochondrial mechanism”. Am J Cardiovasc Drugs 8 (6): 373-418





Ordering information

TechnologyCat. No.ProductContents





Real-Time PCR


SQH01-K020 (20 reaction)





DiaPlexQ ™ ApoE Genotyping Kit
2X Multiplex Real-Time PCR Smart mix (ApoE)
Primer & Probe Mixture (ApoE 112)
Primer & Probe Mixture (ApoE 158)
T/T type Control DNA (ApoE 112)
T/C type Control DNA (ApoE 112)
C/C type Control DNA (ApoE 112)
T/T type Control DNA (ApoE 158)
T/C type Control DNA (ApoE 158)
C/C type Control DNA (ApoE 158)
Nuclease free Water

SQH01-K100 (100 reaction)




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