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Product Name DiaPlexC™ Influenza Virus A Subtype Detection Kit (Respiratory Disease)
Model
Series Molecular Diagnostic (Pathogen Detection)
Catalog
Product Description

DiaPlexC™ Influenza Virus A Subtype Detection Kit

Multiplex PCR based assay system for identification of influenza virus A subtype

OneStep Multiplex RT-PCR

CE-IVD


Influenza virus A (H1N1) is a subtype of Influenza virus A and the most common cause of influenza (flu) in humans. Some strains of H1N1 are endemic in humans and cause a small fraction of all influenza-like illness and a large fraction Of all seasonal influenza. H3N2 is currently endemic in both human and pig populations. It evolved from H2N2 through an antigenic shift and caused the Hong Kong Flu pandemic of 1968 and 1969 that killed almost 750,000 people. The dominant strain of annual flu in January 2006 was H3N2. Since November 2003, nearly 400 cases of human infection with the highly pathogenic Avian Influenza A (H5N1) viruses have been reported by more than a dozen countries in Asia, Africa, the Pacific, Europe and the near East. Highly pathogenic Avian Influenza A (H5N1) virus infections occur in both poultry and humans. Furthermore, although H7N2, H7N3, H7N7 and H9N2 are classified as relatively low pathogenic infections, they have been reported in humans in the past few years and they can potentially develop into pandemic infections.





Specification


DiaPlexC™
Influenza Virus A Subtype Detection kit

Detection targetSet I: H1N1-Seasonal, H1N1-Pandemic, H3N2
Set II: H5N1, H7 common, H9N2
RegistrationCE-IVD
Detection technologyConventional (End-point) OneStep Multiplex RT-PCR
Specimen typeNasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum
Analytical sensitivity10 - 10² copies
Compatible instruments*ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time~ 3 hrs



Features

- Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection

- HotStart PCR system : Ultra high specific and sensitive result

- Reliable system : Automatic PCR control

- Positive control included




Experimental




LaneInterpretation (detection)
1H1N1-Seasonal
2H1N1-Pandemic
3H3N2
4H5N1
5H7 common
6H9N2
7Negative
8NTC





Citation&Papers

Grist NR, Bell EJ, Assaad F.

Enteroviruses in human disease. Prog Med Virol. 1978;24:114-57

Muir P, Nicholson F, Illavia SJ, et al.

Serological and molecular evidence of enterovirus infection in patients with endstage dilated cardiomyopathy. Heart. 1996;76(3):243-9.

Tebruegge M, Curtis N. Enterovirus infections in neonates.

Semin Fetal Neonatal Med. 2009;14(4):222-7.

Imamura T1, Fuji N, Suzuki A, et al.

Enterovirus 68 among children with severe acute respiratory infection, the Philippines. Emerg Infect Dis. 2011;17(8):1430-5.





Ordering information

TechnologyCat. No.ProductContents



Real-Time PCR
SQD41-K020 (20 reaction)

DiaPlexQ™ Influenza Virus A Subtype Detection Kit
OneStep qRT-PCR Enzyme mix (Inf A Sub)
2X OneStep qRT-PCR Buffer (Inf A Sub)
Primer & Probe Mixture I (Inf A Sub)
Primer & Probe Mixture II (Inf A Sub)
Control Template I (Inf A Sub)
Control Template II (Inf A Sub)
RNase free Water
SQD41-K100 (100 reaction)


Conventional (End-point) PCR
SMD41-K020 (20 reaction)

DiaPlexC™ Influenza Virus A Subtype Detection Kit
OneStep RT-PCR Enzyme mix (Inf A Sub)
2X OneStep RT-PCR Buffer (Inf A Sub)
Primer Mixture (Inf A Sub)
Standard Marker (Inf A Sub)
Control Template (Inf A Sub)
RNase free Water
SMD41-K100 (100 reaction)








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