Chat with us, powered by LiveChat [SEF] Solg™ EF-Taq Polymerase (Amplification) | SOLGENT - Komachine
Product Name [SEF] Solg™ EF-Taq Polymerase (Amplification)
Model
Series Research Reagents (PCR)
Catalog
Product Description

[SEF] Solg™ EF-Taq Polymerase

PCR enzyme for long fragment amplification

Long-range PCR

High productivity / Low error rate

Amplicon size : ~ 20 kb


Solg™ EF-Taq polymerase is chemical-mediated HotStart PCR enzyme, which enables multiplex and allele specific PCR for diagnostics and genotyping through initial heat activation during PCR cycling. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.




Specification

SourcePyrococcus furiosus & Thermus aquaticus
(Recombinant)
Amplicon size~ 20 kb
Hot start activityNo
5’→ 3’ exonuclease activityYes3’A or Blunt ends3’A
3’→ 5’ exonuclease activit yYes (weak)Error rate5-6 bp error / 10⁶ bp



Application

- Long-range PCR, High productivity PCR, Inverse PCR, TA cloning




Citation&Papers

Li CS, Zhang QG,


et al., Interleukin-27 polymorphisms are associated with inflammatory bowel diseases in a Korean population, Journal of Gastroenterology and Hepatology , 24 : 1692-1696, 2009

Fu XT, Lin H,


et al., Purification and characterization of a novel β-agarase, AgaA34, from Agarivorans albus YKW-34, Applied Microbiology and Biotechnology , 78 : 265-73, 2008




Format

FormatProduct ImageContentsStorage & Expiry Date
Standard

Solg ™ EF-Taq DNA Polymerase (2.5 U/㎕)
10X EF-Taq Reaction Buffer (25 mM MgCl₂mixed)
10 mM dNTP Mix
5X Band Doctor™
2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)
Smart mix

2X EF-Taq PCR Smar t mix1 year 6 months (In case of Store at -20 ℃ ± 5 ℃)
Pre-Mix

2X EF-Taq PCR Pre-Mix1 year 6 months (In case of Store at -20 ℃ ± 5 ℃)



Ordering information

FormatCat. No.ProductSizePrice(won)
StandardSEF16-R250Solg ™ EF-Taq DNA Polymerase250 U
(200 reaction)
-
SEF16-R500Solg ™ EF-Taq DNA Polymerase500 U-
SEF16-R25hSolg ™ EF-Taq DNA Polymerase2,500 U
(500 U x 5 ea)
-
SEF16-R50hSolg ™ EF-Taq DNA Polymerase5,000 U
(500 U x 10 ea)
-
Smart mixSEF01-M50hSolg ™ 2X EF-Taq PCR Smar t mix 1, with dy e1㎖ x 5 ea
(200 reaction)
-
SEF02-M50hSolg ™ 2X EF-Taq PCR Smar t mix 2 (0.5X B and Doctor), with dye1㎖ x 5 ea-
Pre-MixSEF02-P096Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye,
total volume 30㎕ reaction
96 tube
(8 strip x 12 ea)
-
SEF02-P288Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye,
total volume 30㎕ reaction
288 tube
(8 strip x 36 ea)
-
SEF02-P576Solg ™ 2X EF-Taq PCR Pre-Mix (0.5X Band Doctor), with dye,
total volume 30㎕ reaction
576 tube
(8 strip x 72 ea)
-
Single itemSEF22-B50hDiaStar™ 10X Taq Reaction Buf fer1 ㎖ x 5 ea
(1,000 reaction)
-


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Research Reagents (PCR)
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