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Product Name DiaPlexC™ Apolipoprotein E (ApoE) Genotyping Kit (Alzheimer's Disease)
Model
Series Molecular Diagnostic (Human Gentrifyping)
Catalog
Product Description

DiaPlexC™ Apolipoprotein E (ApoE) Genotyping Kit

Multiplex allele-specific PCR based assay system for the genotyping of the ApoE gene SNP related to alzheimer’s disease

CE-IVD


Apolipoprotein E (ApoE) consists of three isoforms: E2, E3 and E4. These three isoforms make six genotypes, (homozygous genotypes: E2/E2, E3/E3 E4/E4 and heterozygous genotypes: E2/E3, E2/E4, E3/E4). Isoform E2 is usually known as a protective isoform whereas isoform E4 is generally responsible for diseases. The predominant abnormalities are due to a single nucleotide polymorphism (SNP) of codon 112 (Cys/Arg) and codon 158 (Arg/Cys). The polymorphism has functional effects on the lipoprotein metabolism. ApoE is the primary ligand for two receptors, LDL receptors found in the liver and other tissues and ApoE specific receptors found in the liver. The co-ordinate interaction of these lipoprotein complexes with their receptors forms the basis for the metabolic regulation of cholesterol. ApoE has been found to be associated with elevated cholesterol levels or lipid derangements that lead to various clinical problems like coronary heart diseases, strokes, periphery artery diseases, and diabetes mellitus. In addition to the genotype-phenotype associations with the cardiovascular disease, the alleles and isoforms of ApoE have been related to dementias, most commonly Alzheimer’s disease. Determining the genotype of ApoE is thus considered to be an important test for patients with the disease, as well as a precautionary test for healthy people.




Specification


DiaPlexC™ Apolipoprotein E (ApoE) Genotyping Kit
Detection targetApoE genotype (Mutation T112C and T158C of ApoE gene) E2/E2, E3/E3, E4/E4, E2/E3, E2/E4, E3/E4
RegistrationCE-IVD
Detection technologyConventional (End-point) Multiplex PCR
Specimen typeBlood
Compatible instruments*ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time~ 1 hr 30 min




Features

- HotStart PCR system : Ultra high specific and sensitive result

- UDG system : No carryover contamination ( DiaPlexQ™)

- Multiplex PCR : Multiple targets in a single reaction

- Reliable system : Automatic Internal control ( DiaPlexC™)

- Positive control included

- Easy-to-use master mix

- CE certification





Experimental







Lane

Interpretation (detection)
SNPApoE genotype
1112 TT/158 TTE2/E2
2112 TT/158 CCE3/E3
3112 CC/158 CCE4/E4
4112 TT/158 TCE2/E3
5112 TC/158 TCE2/E4
6112 TC/158 CCE3/E4
7Required re-experiment



Citation&Papers

Lepara O, Valjevac A, Alajbegovic A, Zaciragic A, Nakas-Icindic E (August 2009).

Decreased serum lipids in patients with probable Alzheimer’s disease”. Bosn J Basic Med Sci 9 (3): 215-20.

Golomb BA, Evans MA (2008).

“Statin adverse effects : a review of the literature and evidence for a mitochondrial mechanism”. Am J Cardiovasc Drugs 8 (6): 373-418






Ordering information

TechnologyCat. No.ProductContents


Conventional (End-point) PCR
SHG01-K020 (20 reaction)
DiaPlexC™ Apolipoprotein E (ApoE) Genotyping Kit
2X Multiplex PCR Smart mix (ApoE)
Primer Mixture (ApoE)
Standard Marker (ApoE)
Control Template (ApoE)
Nuclease free Water
SHG01-K100 (100 reaction)





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