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Product Name DiaPlexQ™ MERS Virus Detection Kit (Respiratory Disease)
Model
Series Molecular Diagnostic (Pathogen Detection)
Catalog
Product Description

DiaPlexQ™ MERS Virus Detection Kit

Real-Time OneStep RT-PCR based assay system for detection of MERS-CoV

RUO (Research Use Only)


MERS (Middle East respiratory syndrome) is an respiratory virus which was detected from Saudi Arabia on 24th Sep, 2012. The factor virus is MERS-CoV. People suspect that cause of virus come from bat and spread the infection to other animals. The symptom shows very similar with SAS that disease coming from same coronavirus origin. Initial symptom can be distinguish such as influenza because the symptom is very similar with is also. So just symptom diagnostic is really difficult. However, Mers has low infectivity so we can consider possibility of overwhelming disease is kind of low at this moment. But we need to preventive measures against the MERS because virus can be mutate and possible to being highly contagious disease. We should prepare strict prevent measures for early infection block. There is no medicine development yet for MERS virus so far.




Specification



DiaPlexQ™ MERS Virus Detection Kit II (upE/ORF1a/ORF1b)

Detection targetupE, ORF1a, ORF1b
RegistrationRUO
Detection technologyReal-Time OneStep RT-PCR
Specimen type*Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum
Analytical sensitivity10 copies
Compatible instruments**ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems)
CFX96™ Real-Time PCR System (Bio-Rad)
PCR running time

~ 2 hrs





Features

- Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection

- HotStart PCR system : Ultra high specific and sensitive result

- UDG system : No carryover contamination

- Reliable system : Automatic PCR control

- Positive control included




Experimental






Citation&Papers

Victor Max Corman, Ndapewa Laudika Ithete, et al.

Rooting the Phylogenetic Tree of Middle East Respiratory Syndrome Coronavirus by Characterization of a Conspecific Virus from an African Bat. Journal of Virology. 2014;88 (19):1129711303

WHO guidelines for investigation of cases of human infection with Middle East Respiratory

Syndrome Coronavirus(MERS-CoV). WHO. 2013

Novel Coronavirus 2012 Real-Time

RT-PCR Assay (Instructions for Use - International Ver. 001). CDC. 2012





Ordering information

TechnologyCat. No.ProductContents
Real-Time PCRSQD53-K020 (20 reaction)DiaPlexQ ™ MERS Virus Detection Kit I (upE)OneStep qRT-PCR Enzyme mix (MERS)
2X OneStep qRT-PCR Buffer (MERS)
Primer & Probe Mixture (upE)
Control Template (upE)
RNase free Water
SQD53-K100 (100 reaction)
SQD54-K020 (20 reaction)DiaPlexQ ™ MERS Virus Detection Kit II (upE/ORF1a/ORF1b)OneStep qRT-PCR Enzyme mix (MERS)
2X OneStep qRT-PCR Buffer (MERS)
Primer & Probe Mixture (upE/ORF1a/ORF1b)
Control Template (upE/ORF1a/ORF1b)
RNase free Water
SQD54-K100 (100 reaction)
Products of SOLGENT
Molecular Diagnostic (Pathogen Detection)
Research Reagents (PCR)