SOLGENT
SOLGENT
DiaPlexQ™ MERS Virus Detection Kit (Respiratory Disease)
Model
Series
Molecular Diagnostic (Pathogen Detection)
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Description
DiaPlexQ™ MERS Virus Detection Kit
Real-Time OneStep RT-PCR based assay system for detection of MERS-CoV
RUO (Research Use Only)
MERS (Middle East respiratory syndrome) is an respiratory virus which was detected from Saudi Arabia on 24th Sep, 2012. The factor virus is MERS-CoV. People suspect that cause of virus come from bat and spread the infection to other animals. The symptom shows very similar with SAS that disease coming from same coronavirus origin. Initial symptom can be distinguish such as influenza because the symptom is very similar with is also. So just symptom diagnostic is really difficult. However, Mers has low infectivity so we can consider possibility of overwhelming disease is kind of low at this moment. But we need to preventive measures against the MERS because virus can be mutate and possible to being highly contagious disease. We should prepare strict prevent measures for early infection block. There is no medicine development yet for MERS virus so far.
Specification
DiaPlexQ™ MERS Virus Detection Kit II (upE/ORF1a/ORF1b) | ||
|---|---|---|
| Detection target | upE, ORF1a, ORF1b | |
| Registration | RUO | |
| Detection technology | Real-Time OneStep RT-PCR | |
| Specimen type* | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum | |
| Analytical sensitivity | 10 copies | |
| Compatible instruments** | ABI 7500 / 7500 Fast Real-Time PCR System (Applied Biosystems) CFX96™ Real-Time PCR System (Bio-Rad) | |
| PCR running time | ~ 2 hrs | |
Features
- Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection
- HotStart PCR system : Ultra high specific and sensitive result
- UDG system : No carryover contamination
- Reliable system : Automatic PCR control
- Positive control included
Experimental
Citation&Papers
Victor Max Corman, Ndapewa Laudika Ithete, et al. Rooting the Phylogenetic Tree of Middle East Respiratory Syndrome Coronavirus by Characterization of a Conspecific Virus from an African Bat. Journal of Virology. 2014;88 (19):1129711303 | WHO guidelines for investigation of cases of human infection with Middle East Respiratory Syndrome Coronavirus(MERS-CoV). WHO. 2013 | Novel Coronavirus 2012 Real-Time RT-PCR Assay (Instructions for Use - International Ver. 001). CDC. 2012 |
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
| Real-Time PCR | SQD53-K020 (20 reaction) | DiaPlexQ ™ MERS Virus Detection Kit I (upE) | OneStep qRT-PCR Enzyme mix (MERS) 2X OneStep qRT-PCR Buffer (MERS) Primer & Probe Mixture (upE) Control Template (upE) RNase free Water |
| SQD53-K100 (100 reaction) | |||
| SQD54-K020 (20 reaction) | DiaPlexQ ™ MERS Virus Detection Kit II (upE/ORF1a/ORF1b) | OneStep qRT-PCR Enzyme mix (MERS) 2X OneStep qRT-PCR Buffer (MERS) Primer & Probe Mixture (upE/ORF1a/ORF1b) Control Template (upE/ORF1a/ORF1b) RNase free Water | |
| SQD54-K100 (100 reaction) |