SOLGENT
SOLGENT
DiaPlexC™ RV13 Detection Kit (Respiratory Disease)
Model
Series
Molecular Diagnostic (Pathogen Detection)
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Description
DiaPlexC™ RV13 Detection Kit
OneStep Multiplex RT-PCR based assay system for simultaneous detection of 13 major respiratory viruses
CE-IVD
Viral infections are among the leading causes of respiratory disease in children. Most of these infections are caused By respiratory syncyrial virus(RSV), influenza virus A or B (Inf A or Inf B), parainfluenza virus(hPIV-I, II and III), rhinovirus (RV) or adenovirus (AdV). Several recently discovered viruses, such as human metapneumovirus (hMPV), human bocavirus (hBoV), Enterovirus(EV) and the human coronaviruses (hCoVs) 229E and OC43, have been identified as potential respiratory pathogens.
Specification
| DiaPlexC™ RV13 Detection Kit | ||
|---|---|---|
| Detection target | Set I : Metapneumovirus (MPV) Coronavirus OC43(CoV OC43) Coronavirus 229E (CoV 229E) Enterovirus (EntV) Parainfluenza - Ⅰ (PIV-Ⅱ) Parainfluenza - Ⅱ (PIV-Ⅱ) Parainfluenza - Ⅲ (PIV-Ⅲ) Set II : Respiratory syncytial virus (RSV A & B) Adenovirus (AdV) Bocavirus (BoV) Rhinovirus (RV A/B/C) Influenza Virus A (Inf A) Influenza Virus B (Inf B) | |
| Registration | CE-IVD | |
| Detection technology | Conventional (End-point) OneStep Multiplex RT-PCR | |
| Specimen type | Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum | |
| Analytical sensitivity | 10 - 10² copies | |
| Compatible instruments* | ABI Veriti thermal Cycler (Applied Biosystems) recommended | |
| PCR running time | ~ 3 hrs | |
Features
- Simple & Rapid detection system : OneStep Multiplex qRT-PCR based detection
- HotStart PCR system : Ultra high specific and sensitive result
- Reliable system : Automatic PCR control
- Positive control included
- CE certification
Experimental
| Lane | Interpretation (detection) |
|---|---|
| 1 | MPV |
| 2 | CoV 229E |
| 3 | EntV |
| 4 | CoV OC43 |
| 5 | PIV-Ⅲ |
| 6 | PIV-Ⅱ |
| 7 | PIV-Ⅰ |
| 8 | Negative (or NTC) |
| Lane | Interpretation (detection) |
|---|---|
| 1 | RSV A&B |
| 2 | AdV |
| 3 | BoV |
| 4 | RV A/B/C |
| 5 | Inf A |
| 6 | Inf B |
| 7 | Negative (or NTC) |
Citation&Papers
Fouchier RAV, Munster A, Wallenstein TM, Bestebroer S, Herfst D, Smith GF, et al. J Virol. 2002;79:2814-2822 | Lamb RA, Krug R. Field Virology, Lippincott-Raven Philadelphia. 1996; 1353-1395. | Ha Y, Stevens, DJ, Wiley DC. The EMBO Journal. 2002;21;865-875. | Russell RJ, Gamblin SJ, Haire LF, Stevens DJ, Xiao B, Ha Y, et al. Virology. 2004;287-296. |
Ordering information
| Technology | Cat. No. | Product | Contents |
|---|---|---|---|
| Conventional (End-point) PCR | SMD50-K020 (20 reaction) | DiaPlexC ™ RV13 Detection Kit | OneStep RT-PCR Enzyme mix (RV13) 2X OneStep RT-PCR Buffer (RV13) Primer Mixture Ⅰ (RV13) Primer Mixture Ⅱ (RV13) Standard Marker Ⅰ (RV13) Standard Marker Ⅱ (RV13) Control Template Ⅰ(RV13) Control Template Ⅱ (RV13) RNase free Water |
| SMD50-K100 (100 reaction) |