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[SOT] T-Blunt™ PCR Cloning kit (T-Blunt PCR Cloning Kit)
Model
Series
Research Reagents (Cloning)
Transaction Process
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Description
[SOT] T-Blunt™ PCR Cloning kit
PCR Cloning Kit for Sticky & Blunt-end PCR fragment
Cloning of Sticky (A-tailing) & Blunt-end fragment
5-minute cloning Using Topoisomerase
T-Blunt™ PCR Cloning Kit is a suitable for fast cloning of the amplified PCR product by using Taq and Pfu DNA polymerase. By topoisomerase I activation, It enables to do cloning within 5 minutes In the room temperature. With visible selection system in the kit, colony grows only when PCR product is in cloning, so it against unwanted colony by vector self-ligation.
Specification
| T-Blunt™ PCR Cloning Kit | Type of T-Blunt cloning | TA, Blunt | Colony selection system | Antibiotic resistance selectable marker (AmpR, KanR ), ccdB positive selection |
| Insert DNA size | ~ 2 kb | |||
| Cloning enzyme | Topoisomerase I | |||
| Ligation temp. & time | 20 ~ 25 ℃ for 5 min | Sequencing primers | M13(-20) F/R | |
| T-Blunt™ Competent Cell (DH5a) | Type of cells | Chemically competent | Genotype | F-80dlacZ M15 (lacZYA-argF), U169 recA1 endA1 hsdR17(rK-, mK+) phoA supE44 -thi-1 gyrA96 relA1 |
Application
- Blunt-end cloning, TA cloning
Citation&Papers
Park NI, Hui Xu, et al., Anthocyanin Accumulation and Gene Expression of Anthocyanin Biosynthetic Enzymes in Radish (Raphanus sativus), JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 59 : 6034-6039, 2011 | Cho SJ, Naoki F, et al., Development of a simultaneous partial nitrification and anaerobic ammonia oxidation process in a single reactor, Bioresource Technology, 102 : 652-9, 2011 | Hien TT, Park HD, et al., Influence of Different Substrates in Wetland Soils on Denitrification, WATER AIR AND SOIL POLLUTION , 215 : 549-560, 2011 |
Format
| Format | Product image | Contents | Storage & Expiry date |
|---|---|---|---|
| T-Blunt™ PCR Cloning Kit |   | T-Blunt vector 6X T-Blunt buffer M13F(-20) / M13R(-20) primer (10 pmole/㎕) Control insert (880 bp) (20 ng/㎕) | 6 months (In case of Store at -20 ℃ ± 5 ℃) |
| T-Blunt™ Competent Cell (DH5a) |  | DH5αcompetent cell pUC19 control plasmid (10 pg/㎕) S.O.C 배지 | 6 months (In case of Store at -70 ℃) |
Ordering information
| Cat. No. | Product | Size | Price(won) |
|---|---|---|---|
| SOT01-K020 | T-Blunt™ PCR Cloning Kit (T-Blunt vector, Buffer, Competent cell) | 20 reaction | - |
| SOT02-K020 | T-Blunt™ PCR Cloning Kit (T-Blunt vector, Buffer) | 20 reaction | - |
| SOT41-K020 | T-Blunt™ Competent Cell (DH5ɑ) | 20 reaction | - |