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[DR] DiaStar™ RT Series (cDNA Synthesis)

Model

Series

Research Reagents (PCR)


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Description

[DR] DiaStar™ RT Series

For cDNA synthesis with high sensitivity & high efficiency from Template RNA


Diastar™ RT Kit is a thermostable mutant of M-MLV Reverse Transcriptase (Rnase H-) which is recombinant type. This mutant type of Rnase H- enables more efficient cDNA synthesis due to more thermostable characteristic (optimal activity at 50 ℃).



Specification

SourceM-MLV (Recombinant)H Gene-specific primer -~ 14 kb
Reaction temperature42 – 55 ℃Applicable PrimersRandom primer, Oligo d(T) primer, Gene-specific primer



Application

- First-strand cDNA synthesis, Two-Step RT-PCR (conventional and real-time PCR), cDNA library construction, Primer extension analysis




Citation&Papers

Ramasamy H,

Kim MC, et al., Immune response and expression analysis of cathepsin K in goldfish during Aeromonas hydrophila infection, Fish & Shellfish Immunology, 28 : 511-516, 2010

Chen XY, Lin Liu,

et al., The Arabidopsis Callose Synthase Gene GSL8 Is Required for Cytokinesis and Cell Patterning, Plant Physiology, 150 :105-113, 2009





Format

FormatProduct ImageContentsStorage & Expiry Date
Standard

DiaStar™ RTase (200 U/㎕)
5X RT Reaction Buffer (Included 10 mM dNTP mix)
8 mM DTT
(Option) RNase Inhibitor
DiaStar™ RT Kit
: 2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)
RNase Inhibitor
: 1 year 6 months (In case of Store at -20 ℃ ± 5 ℃)
Pre-Mix

2X RT Pre-Mix1 year 6 months (In case of Store at -20 ℃ ± 5 ℃)





Ordering information

FormatCat. No.ProductSizePrice (won)
StandardDR22-R10kDiaStar™ RT Kit, with RNase Inhibitor
(RTase (M-MLV, RNase H-), Reaction Buffer (Included 10 mM dNTP), 8 mM DTT, RNase Inhibitor)
10,000 U
(200 U/㎕)
(50 reaction)
-
DR23-R10kDiaStar™ RT Kit
(RTase (M-MLV, RNase H-), Reaction Buffer (Included 10 mM dNTP), 8 mM DTT)
10,000 U
(200 U/㎕)
-
Pre-MixDR41-P096DiaStar™ 2X RT Pre-Mix, w/o dye, total volume 20 ㎕ reaction96 tube
(8 strip x 12 ea)
-
Single itemDR28-B50hDiaStar™ 5X RT Reaction Buffer (Included 10 mM dNTP)1 ㎖ x 5 ea
(1,250 reaction)
-
SOR51-E20hSolg™ RNase Inhibitor2,000 U
(40 U/㎕)
-
SOR51-E10kSolg™ RNase Inhibitor10,000 U
(40 U/㎕)
-




Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

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