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[SMP] Solg™ 2X Multiplex PCR Series (Amplification)

Model

Series

Research Reagents (PCR)


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Description

[SMP] Solg™ 2X Multiplex PCR Series

For fast Multiplex PCR set-up

Multiplex PCR up to 23-plex

Chemical-mediated Hot start enzyme

Ultra-high specificity


Amplicon size : ~ 1 kb

Solg™ 2X Multiplex PCR is designed to amplify multiple target from single template in single PCR tube using Solg™ h-Taq DNA polymerase. Chemically modified HotStart enzyme, Solg™ h-Taq DNA polymerase is useful for multiplex PCR, genotyping, microsatellite due to its high specificity. Recommended to use of amplification for less than 1 kb PCR product.



Specification


SourceThermus aquaticus
(Solg ™ h-Taq )
Amplicon size~ 1kb
Hot start activityYes(Chemical-mediated)
5’→ 3’ exonuclease activityyes3’A or Blunt ends3’A
3’→ 5’ exonuclease activit yNoError rate12-13 bp error / 10⁶ bp



Application

- Multiplex PCR, Molecular diagnosis, Allele specific PCR, Highly specific amplification of genomic and cDNA tar gets, SNP detection, SSR/MS, TA cloning




Citation&Papers

RYUK JA, CHOI GY,

et al., Application of Genetic Marker and Real-Time Polymerase Chain Reaction for Discrimination between Forsythia viridissima and Forsythia suspensa, Biological & pharmaceutical bulletin, 33 : 1133-7, 2010

Go HA, Lee MJ,

et al., Fast Identification of Korean Cattle Based on Simultaneous Dtection of Various Single Nucleotide Polymorphisms Markers by Capillary Electrophoresis, BULLETIN OF THE KOREAN CHEMICAL SOCIETY , 30 : 2141-2144, 2010

Oh DR, Cheong IC,

et al, Fast Microchip Electrophoresis Using Field Strength Gradients for Single Nucleotide Polymorphism Identification of Cattle Breeds, BULLETIN OF THE KOREAN CHEMICAL SOCIETY, 31 : 1902-1906, 2010





Format

FormatProduct ImageContentsStorage & Expiry Date
Smart mix

2X Multiplex PCR Smart mix1 year 6 months(In case of Store at -20 ℃ ± 5 ℃)
Pre-Mix

2X Multiplex PCR Pre-Mix1 year 6 months(In case of Store at -20 ℃ ± 5 ℃)



Ordering information

Format
Cat. NoProductSizePrice(won)
Smart mixSMP01-M25hSolg ™ 2X Multiplex PCR Smar t mix, with dye0.5 ㎖ x 5 ea
(100 reaction)
-
SMP02-M25hSolg ™ 2X Multiplex PCR Smar t mix, w/o dye0.5 ㎖ x 5 ea-
Pre-MixSMP01-P096Solg ™ 2X Multiplex PCR Pre-Mix, with dye, total volume 30 ㎕ reaction96 tube
(8 strip x 12 ea)
-
SMP02-P096Solg ™ 2X Multiplex PCR Pre-Mix, w/o dye, total volume 30 ㎕ reaction96 tube-




Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

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