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DiaPlexC™ MTB/M.Bovis Detection Kit (Tuberculosis)

Model

Series

Molecular Diagnostic (Pathogen Detection)


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Description

DiaPlexC™ MTB/M.Bovis Detection Kit

Multiplex PCR based assay system for simultaneous detection of MTB and M. bovis among MTC complex

CE-IVD


Mycobacterium tuberculosis (TB) is one of the most ubiquitously found dominant infectious agents around the world especially in developing countries that cause tuberculosis for almost 8 million and the death of 2 million people each year. Though there is a prevention method, the BCG (Bacillus Calmette-Guerin) TB vaccination, its disadvantage is that it cannot protect after 5 years from immunization and there is also possibility of diagnostic error for actual TB because there is a high chance of a false-positive result in the tuberculin test.



Specification


DiaPlexC™ MTB/M. Bovis Detection Kit
Detection targetMycobacterium tuberculosis (MTB), Mycobacterium bovis (M. bovis)
RegistrationCE-IVD
Detection technologyConventional (End-point) Multiplex PCR
Specimen type
Nasopharyngeal swab, Nasopharyngeal aspirate, Bronchoalveolar lavage (BAL), Nasal swab, Oropharyngeal swab, Sputum
Analytical sensitivity10² copies
Compatible instruments*ABI Veriti thermal Cycler (Applied Biosystems) recommended
PCR running time~ 2 hrs



Features

- HotStart PCR system : ultra high specific and sensitive result

- DnaFree™ system : No host genomic DNA contamination

- UDG system : No carryover contamination

- Multiplex PCR system : Multiple targets in a single reaction

- Reliable system : Automatic PCR control

- Positive control included

- Easy-to-use master mix

- CE certification




Experimental


LaneInterpretation (detection)
1Negative (or NTC)
2MTB
3M. bovis
4MTB, M. bovis (Co-infection)
5Required re-experiment



Citation&Papers

“Mycobacterium tuberculosis” Sanger Institute.

2007-03-29

http://www.sanger.ac.uk/projects/M_tuberculosis/. Retrieved 2008-11-16

Frothingham R, Meeker-Meeker-O’Connell WA. (1998).

“Genetic diversity in the Mycobacterium tuberculosis complex based on variable numbers of tandem DNA repeats.”. Microbiology 144 (Pt 5): 1189-96.

Teo, SS ; Shingadia, DV (2006 Jun).

“Does BCG have a role in tuberculosis control and prevention in the United Kingdom?”. Archives of Disease in Childhood 91 (6) : 529-31.





Ordering information

TechnologyCat. No.ProductContents
Conventional (End-point) PCRSMD22-K020 (20 reaction)DiaPlexC ™ MTB/M. Bovis Detection Kit2X Multiplex PCR Smart mix (with UDG)
(MTB/M. Bovis)
Primer Mixture (MTB/M. Bovis)
Standard Marker (MTB/M. Bovis)
Control Template (MTB/M. Bovis)
Nuclease free Water
SMD22-K100 (100 reaction)





Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

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