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[SPX] Solg™ Pfu-X DNA Polymerase (Amplification)

Model

Series

Research Reagents (PCR)


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Description

[SPX] Solg™ Pfu-X DNA Polymerase

For blunt-end PCR fragment amplification with fast speed & high accuracy

Ultra-high fidelity Long-range PCR

Fast elongation rate

Amplicon size : ~ 20 kb


Solg™ Pfu-X DNA polymerase is an upgraded version of Pfu DNA polymerase for long-range and high fidelity PCR. Processivity and Fidelity of Pfu-X DNA polymerase is 4 times faster and 3 times higher than Pfu polymerase. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.




Specification

SourcePyrococcus furiosus
(Recombinant)
Amplicon size~ 20 kb
Hot start activityNo
5’→ 3’ exonuclease activityNo3’A or Blunt endsBlunt
3’→ 5’ exonuclease activit yYesError rate1-2 bp error / 10⁶ bp



Application

- Long-range PCR, High fidelity PCR, Site-directed mutagenesis, Blunt-end cloning




Format


FormatProduct ImageContentsStorage & Expiry Date
Standard

Solg ™ Pfu-X DNA Polymerase (2.5 U/㎕)
10X Pfu-X Reaction Buffer (25 mM MgCl₂mixed)
10 mM dNTP Mix
5X Band Doctor™
2 years 3 months(In case of Store at -20 ℃ ± 5 ℃)





Ordering information

Format
Cat. No.
Product
Size
Price(won)
StandardSPX16-R250Solg ™ Pfu-X DNA Polymerase250 U
(200 reaction)
-
SPX16-R500Solg ™ Pfu-X DNA Polymerase500 U-
SPX16-R25hSolg ™ Pfu-X DNA Polymerase2,500 U
(500 U x 5 ea)
-
SPX16-R50hSolg ™ Pfu-X DNA Polymerase5,000 U
(500 U x 10 ea)
-
Single itemSPX22-B50hSolg ™ 10X Pfu-X Reaction Buffer1 ㎖ x 5 ea
(1,000 reaction)
-




Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

ⓒ2024 Komachine Inc. All rights reserved.