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[SHT] Solg™ h-Taq DNA Polymerase (Amplification)

Model

Series

Research Reagents (PCR)


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Description

[SHT] Solg™ h-Taq DNA Polymerase

For High specific PCR amplification

Genotyping

Chemical-mediated Hot start enzyme

Ultra-high specificity

Amplicon size : ~ 1 kb


Solg™ h-Taq polymerase is chemically modified HotStart PCR enzyme, which enables multiplex and allele specific PCR for diagnostics and genotyping through initial heat activation during PCR cycling. You may modify the amount of template, extension time, annealing temperature, amount of Taq DNA polymerase, the number of PCR cycle, and amount of 5X Band Doctor™ according to the target size, primer’s Tm, and the type of templates for amplification.




Specification

SourceThermus aquaticus
(Recombinant)
Amplicon size~ 1 kb
Hot start activityYes(Chemical-mediated)
5’→ 3’ exonuclease activityYes3’A or Blunt ends3’A
3’→ 5’ exonuclease activit yNoError rate12-13 bp error / 10⁶ bp




Application

Genotyping, Allele specific PCR, Highly specific amplification of genomic and cDNA targets, High G+C rich / Repeat region PCR, TA cloning




Citation&Papers

Lee GA, Kwon SJ,


et al., Cross-amplification of SSR markers developed from Allium sativum to other Allium species, Scientia Horticulturae , 128 : 401-407, 2011

Kee SK, Lee JY,


et al., Hypermethylation of the Ras Association Domain Family 1A (RASSF1A) Gene in Gallbladder Cancer, Molecules and Cells , 24 : 364-371, 2007

Lee HJ, Cha KE,


et al., In voitr Screening System for Hepatotoxicity: Comparison of Bone-Marrow-Derived Mesenchymal Stem Cells and Placenta-Derived Stem Cells,J ournal of Cellular Biochemistry, 112 : 49-58, 2011




Format

FormatProduct ImageContentsStorage & Expiry Date
Standard

Solg ™ h-Taq DNA Polymerase (2.5 U/㎕)
10X h-Taq Reaction Buffer (15 mM MgCl₂mixed)
10 mM dNTP Mix
5X Band Doctor™
2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)
Smart mix

2X h-Taq PCR Smar t mix2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)
Pre-Mix

2X h-Taq PCR Pre-Mix1 year 6 months (In case of Store at -20 ℃ ± 5 ℃)



Ordering information

FormatCat. No.ProductSizePrice(won)
StandardSHT06-R250Solg ™ h-Taq DNA Polymerase250 U
(200 reaction)
-
SHT06-R10hSolg ™ h-Taq DNA Polymerase1,000 U
(250 U x 4 ea)
-
SHT06-R25hSolg ™ h-Taq DNA Polymerase2,500 U
(250 U x 10 ea)
-
SHT06-R50hSolg ™ h-Taq DNA Polymerase5,000 U
(250 U x 20 ea)
-
Smart mixSHT01-M40hSolg ™ 2X h-Taq PCR Smar t mix 1, with dye1 ㎖ x 4 ea
(160 reaction)
-
SHT03-M40hSolg ™ 2X h-Taq PCR Smar t mix 2 (1.0X B and Doctor), with dye1 ㎖x 4 ea-
Pre-MixSHT01-P096Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction96 tube
(8 strip x 12 ea)
-
SHT01-P288Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction288 tube
(8 strip x 36 ea)
-
SHT01-P576Solg ™ 2X h-Taq PCR Pre-Mix, with dye, total volume 30 ㎕ reaction576 tube
(8 strip x 72 ea)
-
Single itemSHT21-B50hSolg ™ 10X h-Taq Reaction Buffer1 ㎖ x 5 ea
(1,000 reaction)
-
SHT95-E250Solg™ h-Taq DNA Polymerase (only enzyme)250 U
-





Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

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