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[DE] DiaStar™ EF-Taq DNA Polymerase (Amplification)

Model

Series

Research Reagents (PCR)


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Description

[DE] DiaStar™ EF-Taq DNA Polymerase

Hot start PCR enzyme for high sensitivity & high efficiency & long fragment amplification

Long-range PCR

Antibody-mediated Hot start enzyme

High sensitivity & productivity / Low error rate

Amplicon size : ~ 20 kb


DiaStar™ EF-Taq DNA polymerase is antibody mediated HotStart DNA polymerase which is good for long-range PCR by mixing Taq DNA polymerase and Pfu DNA polymerase. It is recommended to use it when amplifying 40Kb or less.




Specification

SourcePyrococcus furiosus & Thermus aquaticus
(Recombinant)
Amplicon size~ 20 kb
Hot start activityYes (Antibody-mediated)
5’→ 3’ exonuclease activityYes3’A or Blunt ends3’A
3’→ 5’ exonuclease activit yYes (weak)Error rate5-6 bp error / 10⁶ bp




Application

- Long-range PCR, Amplification of low-c opy DNA targets, High throughput hot start PCR, Highly specific amplification of genomic and cDNA tar gets, Inverse PCR, TA cloning





Format

FormatProduct ImageContentsStorage & Expiry Date
StandardDiaStar™ EF-Taq DNA Polymerase (2.5 U/㎕)
10X EF-Taq Reaction Buffer (25 mM MgCl₂mixed)
10 mM dNTP Mix
5X Band Doctor™
2 years 3 months (In case of Store at -20 ℃ ± 5 ℃)



Ordering information

FormatCat. No.ProductSizePrice(won)
StandardDE16-R250DiaStar™ EF-Taq DNA Polymerase250 U
(200 reaction)
-
DE16-R500DiaStar™ EF-Taq DNA Polymerase500 U-
DE16-R25hDiaStar™ EF-Taq DNA Polymerase2,500 U
(500 U x 5 ea)
-
DE16-R50hDiaStar™ EF-Taq DNA Polymerase5,000 U
(500 U x 10 ea)
-
Single itemDE22-B50hDiaStar™ 10X EF-Taq Reaction Buffer1 ㎖ x 5 ea
(1,000 reaction)
-





Inquiry

Komachine Inc.
Head Office:
Ace Dongbaek Tower 1-1101, 16-4,
Dongbaekjungang-ro 16beon-gil, Giheung-gu,
Yongin City, Gyeonggido, South Korea 17015
Branch Office:
606, Seoul Stratup Center, 10,
Noryangjin-ro, Dongjak-gu, Seoul, South Korea 06938
CEOCharlie Park
Corporate #535-86-00664

ⓒ2024 Komachine Inc. All rights reserved.